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Differential abundance analysis was performed on <t>16S</t> rRNA gene sequencing data from recipient flies following FMT. (A) Coefficient plot showing differential abundance of selected bacterial taxa across donor–recipient combinations following FMT. Positive coefficient values indicate relative enrichment, whereas negative values indicate relative depletion. (B–C) Relative abundance of Lactobacillus plantarum (B) and Lactobacillus pentosus–plantarum (C) in control and Kdm5 LOF recipient flies following FMT. Individual points represent biological replicates. Each condition represents three independent biological replicates. Statistical significance was determined using differential abundance analysis with false discovery rate (FDR) correction. Adjusted p-values and coefficients are indicated where significant.
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Workflow for laboratory detection and AST of A. baumannii in RTE foods. Isolates were recovered by culture and biochemistry, screened by VITEK-2 Compact, identified by <t>MALDI-TOF</t> MS and real-time polymerase chain reaction (RT-PCR), and tested for antimicrobial susceptibility using Kirby Bauer disk diffusion and VITEK-2, with colistin by broth microdilution. Composite species identity required either MALDI-TOF MS species-level scoring or bla OXA-51-like RT-PCR positivity.
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Differential abundance analysis was performed on 16S rRNA gene sequencing data from recipient flies following FMT. (A) Coefficient plot showing differential abundance of selected bacterial taxa across donor–recipient combinations following FMT. Positive coefficient values indicate relative enrichment, whereas negative values indicate relative depletion. (B–C) Relative abundance of Lactobacillus plantarum (B) and Lactobacillus pentosus–plantarum (C) in control and Kdm5 LOF recipient flies following FMT. Individual points represent biological replicates. Each condition represents three independent biological replicates. Statistical significance was determined using differential abundance analysis with false discovery rate (FDR) correction. Adjusted p-values and coefficients are indicated where significant.

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Differential abundance analysis was performed on 16S rRNA gene sequencing data from recipient flies following FMT. (A) Coefficient plot showing differential abundance of selected bacterial taxa across donor–recipient combinations following FMT. Positive coefficient values indicate relative enrichment, whereas negative values indicate relative depletion. (B–C) Relative abundance of Lactobacillus plantarum (B) and Lactobacillus pentosus–plantarum (C) in control and Kdm5 LOF recipient flies following FMT. Individual points represent biological replicates. Each condition represents three independent biological replicates. Statistical significance was determined using differential abundance analysis with false discovery rate (FDR) correction. Adjusted p-values and coefficients are indicated where significant.

Article Snippet: Taxonomic assignment was performed using UCLUST within QIIME v1.9.1, using the Zymo Research curated 16S reference database.

Techniques: Sequencing, Control

Workflow for laboratory detection and AST of A. baumannii in RTE foods. Isolates were recovered by culture and biochemistry, screened by VITEK-2 Compact, identified by MALDI-TOF MS and real-time polymerase chain reaction (RT-PCR), and tested for antimicrobial susceptibility using Kirby Bauer disk diffusion and VITEK-2, with colistin by broth microdilution. Composite species identity required either MALDI-TOF MS species-level scoring or bla OXA-51-like RT-PCR positivity.

Journal: Pathogens

Article Title: Antimicrobial Resistance in Acinetobacter baumannii Isolated from Ready-to-Eat Foods in Saudi Arabia

doi: 10.3390/pathogens15030261

Figure Lengend Snippet: Workflow for laboratory detection and AST of A. baumannii in RTE foods. Isolates were recovered by culture and biochemistry, screened by VITEK-2 Compact, identified by MALDI-TOF MS and real-time polymerase chain reaction (RT-PCR), and tested for antimicrobial susceptibility using Kirby Bauer disk diffusion and VITEK-2, with colistin by broth microdilution. Composite species identity required either MALDI-TOF MS species-level scoring or bla OXA-51-like RT-PCR positivity.

Article Snippet: Spectra were matched against the Bruker MALDI Biotyper reference database [ , , ].

Techniques: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Diffusion-based Assay